Associate Scientist III, Mechanistic Cell Biology

insitroOnsite

Job Functions1. Designing, validating and performing biochemical and cellbased assays2. Analyzing/interpreting data to expand understanding of target engagement and downstream signaling pathways of multiple targets across a variety of therapeutic modalities3. Building and optimizing transient and stable reporter cell lines (luciferase and fluorescencebased)4. Maintaining cultures of immortalized and primary cell lines and work with difficult cell models including iPSCs to perform biobanking, cell differentiation and cellbased assays involving genetic manipulation using siRNA and/or CRISPR5. Working crossfunctionally with scientists from diverse therapeutics areas and functions at Insitro to help answer mechanistic questions and to support assay development, experimental design, troubleshooting, sample analysis, data interpretation, and report generation.

Job Requirements1. Master's degree in Biotechnology, Biochemistry, Cell Biology or a related field plus two (2) years of experience in any occupation which includes the required experience/skills.2. Bachelor's degree in Biotechnology, Biochemistry, Cell Biology or a related field plus five (5) years of progressive experience in any related occupation.

Skills1. Developing, optimizing and performing endpoint and image-based assays in 96- and 384-well plate formats (or other high-throughput formats) including ELISA assays and Luminex multiplex assays using cell and tissue samples2. Transient and stable cell line reporter generation and using a wide range of molecular biology techniques including cloning, plasmid purification, gene editing, qPCR (Quantstudio), RNA isolation and protein quantification by western blot3. Immunophenotyping, cell sorting and development of functional assays using advanced flow cytometry techniques (8+ color panels)4. Utilizing aseptic technique for cell culture and handling immortalized cell lines, primary cells, iPSCs, as well as processing of rodent tissue and plasma samples from in vivo studies5. GraphPad Prism, Benchling and FlowJo (flow cytometry analysis software) software1. Strong communication skills to work crossfunctionally with scientists from diverse therapeutics areas and functions at Insitro2. Ability to troubleshoot issues and think critically to solve problems3. Collaborative mindset to work as part of a team towards common goals4. Attention to detail in data analysis and interpretation

Associate Scientist III, Mechanistic Cell Biology wanted by Insitro, Inc. in South San Francisco, CA. Designing, validating and performing biochemical and cell-based assays, as well as analyze/interpret data, to expand understanding of target engagement and downstream signaling pathways of multiple targets across a variety of therapeutic modalities; Building and optimizing transient and stable reporter cell lines (luciferase and fluorescence-based) to generate reliable and robust screening assays for drug candidates; Maintaining cultures of immortalized and primary cell lines and work with difficult cell models including iPSCs to perform biobanking, cell differentiation and cell-based assays involving genetic manipulation using siRNA and/or CRISPR, as well as handling and processing of tissue and plasma samples from in vivo studies; Working cross-functionally with scientists from diverse therapeutics areas and functions at Insitro to help answer mechanistic questions and to support assay development, experimental design, troubleshooting, sample analysis, data interpretation, and report generation.

Must have a Master's degree in Biotechnology, Biochemistry, Cell Biology or a related field plus two (2) years of experience in any occupation which includes the required experience/skills. Must also have two (2) years of experience developing, optimizing and performing end-point and image-based assays in 96- and 384-well plate formats (or other high-throughput formats) including ELISA assays and Luminex multiplex assays using cell and tissue samples; two (2) years of experience with transient and stable cell line reporter generation and using a wide range of molecular biology techniques including cloning, plasmid purification, gene editing, qPCR (Quantstudio), RNA isolation and protein quantification by western blot; two (2) years of experience with immunophenotyping, cell sorting and development of functional assays using advanced flow cytometry techniques (8+ color panels); two (2) years of experience utilizing aseptic technique for cell culture and handling immortalized cell lines, primary cells, iPSCs, as well as processing of rodent tissue and plasma samples; and two (2) years of experience with GraphPad Prism, Benchling and FlowJo (flow cytometry analysis software) software.

In lieu of a Master's degree plus two (2) years of experience, we will accept a Bachelor's degree in Biotechnology, Biochemistry, Cell Biology or a related field plus five (5) years of progressive experience in any related occupation. Must also have three (3) years of experience developing, optimizing and performing end-point and image-based assays in 96- and 384-well plate formats (or other high-throughput formats) including ELISA assays and Luminex multiplex assays using cell and tissue samples; two (2) years of experience with transient and stable cell line reporter generation and using a wide range of molecular biology techniques including cloning, plasmid purification, gene editing, qPCR (Quantstudio), RNA isolation and protein quantification by western blot; three (3) years of experience with immunophenotyping, cell sorting and development of functional assays using advanced flow cytometry techniques (8+ color panels); two (2) years of experience utilizing aseptic technique for cell culture and handling immortalized cell lines, primary cells, iPSCs, as well as processing of rodent tissue and plasma samples; and two (2) years of experience with GraphPad Prism, Benchling and FlowJo (flow cytometry analysis software) software. $112,258-$125,000. Please send resume to: ta-resumes@insitro.com and refer to: Job Req. 2025-insitro-001P

Compensation & Benefits at insitro

Our target starting salary for successful US-based applicants for this role is $112,258-$125,000. To determine starting pay, we consider multiple job-related factors including a candidate's skills, education and experience, market demand, business needs, and internal parity. We may also adjust this range in the future based on market data.This role is eligible for participation in our Annual Performance Bonus Plan (based on company targets by role level and annual company performance) and our Equity Incentive Plan, subject to the terms of those plans and associated policies.In addition, insitro also provides our employees:

401(k) plan with employer matching for contributions
Excellent medical, dental, and vision coverage (insitro pays 100% of premiums for employees on our base plans), as well as mental health and well-being support
Open, flexible vacation policy
Paid parental leave
Quarterly budget for books and online courses for self-development
Support to occasionally attend professional conferences that are meaningful to your career growth and development
New hire stipend for home office setup
Monthly cell phone & internet stipend
Access to free onsite baristas and cafe with daily lunch and breakfast
Access to free onsite fitness center
Commuter benefits

insitro is an equal opportunity employer. All applicants will be considered for employment without attention to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran or disability status.We believe diversity, equity, and inclusion need to be at the foundation of our culture. We work hard to bring together diverse teams–grounded in a wide range of expertise and life experiences–and work even harder to ensure those teams thrive in inclusive, growth-oriented environments supported by equitable company and team practices.

All candidates can expect equitable treatment, respect, and fairness throughout the interview process.

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About insitro

insitro is a drug discovery and development company using machine learning (ML) and data at scale to decode biology for transformative medicines. At the core of insitro’s approach is the convergence of in-house generated multi-modal cellular data and high-content phenotypic human cohort data. We rely on these data to develop ML-driven, predictive disease models that uncover underlying biologic state and elucidate critical drivers of disease. These powerful models rely on extensive biological and computational infrastructure and allow insitro to advance novel targets and patient biomarkers, design therapeutics and inform clinical strategy.

insitro is advancing a wholly owned and partnered pipeline of insights and therapeutics in neuroscience, oncology and metabolism. Since launching in 2018, insitro has raised over $700 million from top tech, biotech and crossover investors, and from collaborations with pharmaceutical partners. For more information on insitro, please visit www.insitro.com.

Life at insitro

insitro is a data-driven drug discovery and development company that leverages machine learning and high-throughput biology to transform the way medicines are created to help patients. At insitro, we are rethinking the entire drug discovery process, from the perspective of machine learning, human genetics, and high-throughput, quantitative biology. Over the past five decades, we have seen the development of new medicines becoming increasingly more difficult and expensive, leaving many patients with significant unmet need. Weￃﾢ￢ﾂﾬ￢ﾄﾢre embarking on a new approach to drug development ￃﾢ￢ﾂﾬ￢ﾀﾜ one that leverages machine learning and unique in vitro strategies for modeling disease state and designing new therapeutic interventions. We aim to eliminate key bottlenecks in traditional drug discovery, so we can help more people sooner and at a much lower cost to the patient and the healthcare industry. We believe that by harnessing the power of technology to interrogate and measure human biology, we can have a major impact on many diseases. We invest heavily in cutting edge bioengineering technologies to enable the construction of large-scale, high-quality data sets that are designed specifically to drive machine learning methods. Our first application is to use human genetics, functional genomics, and machine learning to build a new generation of in vitro human cell-derived disease models whose response to perturbation is designed to be predictive of human clinical outcomes. This cannot be done without great people. We are bringing together an outstanding team of people whose expertise spans multiple disciplines - life sciences, machine learning, human genetics, engineering, and drug discovery - and building a unique culture where people from diverse backgrounds work as a single team towards a common goal. We offer opportunities to collaborative people with expertise in life science and computational science. Join us to help bring better health to more people, faster and cheaper.

Thrive Here & What We Value1. Inclusive, growth-oriented environment supported by equitable company and team practices2. Open, flexible vacation policy3. Paid parental leave4. Quarterly budget for books and online courses for self-development5. Monthly cell phone & internet stipend6. Access to free onsite baristas and cafe with daily lunch and breakfast7. Access to free onsite fitness center8. Commuter benefits9. Equal opportunity employer1e. Commitment to diversity, equity, and inclusion

Associate Scientist III, Mechanistic Cell Biology

Compensation & Benefits at insitro

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About insitro

Life at insitro

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